| Autor: |
de Figueiredo ML; Institute of Biomedical Sciences of the University of Sao Paulo, Sao Paulo, Brazil., de C Gomes A, Amarilla AA, de S Leandro A, de S Orrico A, de Araujo RF, do S M Castro J, Durigon EL, Aquino VH, Figueiredo LT |
| Jazyk: |
angličtina |
| Zdroj: |
Virology journal [Virol J] 2010 Jul 12; Vol. 7, pp. 152. Date of Electronic Publication: 2010 Jul 12. |
| DOI: |
10.1186/1743-422X-7-152 |
| Abstrakt: |
Dengue epidemics have been reported in Brazil since 1985. The scenery has worsened in the last decade because several serotypes are circulating and producing a hyper-endemic situation, with an increase of DHF/DSS cases as well as the number of fatalities. Herein, we report dengue virus surveillance in mosquitoes using a Flavivirus genus-specific RT-Hemi-Nested-PCR assay. The mosquitoes (Culicidae, n = 1700) collected in the Northeast, Southeast and South of Brazil, between 1999 and 2005, were grouped into 154 pools. Putative genomes of DENV-1, -2 and -3 were detected in 6 mosquito pools (3.8%). One amplicon of putative DENV-1 was detected in a pool of Haemagogus leucocelaenus suggesting that this virus could be involved in a sylvatic cycle. DENV-3 was found infecting 3 pools of larvae of Aedes albopictus and the nucleotide sequence of one of these viruses was identified as DENV-3 of genotype III, phylogenetically related to other DENV-3 isolated in Brazil. This is the first report of a nucleotide sequence of DENV-3 from larvae of Aedes albopictus. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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