| Autor: |
Abianova AR, Chulkin AM, Vavilova EA, Fedorova TV, Loginov DS, Koroleva OV, Benevolenskiĭ SV |
| Jazyk: |
ruština |
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2010 May-Jun; Vol. 46 (3), pp. 342-7. |
| Abstrakt: |
A heterologous protein expression in the fungus Penicillium canescens is described for the first time. The fungal strains producing Trametes hirsuta laccase under control of a highly efficient promoter of the P. canescens gene bgaS has been constructed. These strains efficiently transcribe the T. hirsuta 072 laccase gene with a correct intron splicing. Activity of the secreted heterologous laccase in the culture liquid reaches 3 U/ml, accounting for 98% of the total laccase activity, which demonstrates a high efficiency ofheterologous secretion. The synthesized P. canescens laccase has the same molecular weight as the enzyme produced by T. hirsuta 072. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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