Autor: |
Jerdeva GV; Division of Biology, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA., Tesar DB, Huey-Tubman KE, Ladinsky MS, Fraser SE, Bjorkman PJ |
Jazyk: |
angličtina |
Zdroj: |
Traffic (Copenhagen, Denmark) [Traffic] 2010 Sep; Vol. 11 (9), pp. 1205-20. Date of Electronic Publication: 2010 May 26. |
DOI: |
10.1111/j.1600-0854.2010.01083.x |
Abstrakt: |
Protein delivery across polarized epithelia is controlled by receptor-mediated transcytosis. Many studies have examined basolateral-to-apical trafficking of polymeric IgA (pIgA) by the polymeric immunoglobulin receptor (pIgR). Less is known about apical-to-basolateral transcytosis, the direction the neonatal Fc receptor (FcRn) transports maternal IgGs across intestinal epithelia. To compare apical-to-basolateral and basolateral-to-apical transcytosis, we co-expressed FcRn and pIgR in Madin-Darby canine kidney (MDCK) cells and used pulse-chase experiments with confocal microscopy to examine transport of apically applied IgG Fcgamma and basolaterally applied pIgA. Fcgamma and pIgA trafficking routes were initially separate but intermixed at later chase times. Fcgamma was first localized near the apical surface, but became more equally distributed across the cell, consistent with concomitant transcytosis and recycling. By contrast, pIgA transport was strongly unidirectional: pIgA shifted from near the basolateral surface to an apical location with increasing time. Some Fcgamma and pIgA fluorescence colocalized in early (EEA1-positive), recycling (Rab11a-positive), and transferrin (Tf)-positive common/basolateral recycling endosomes. Fcgamma became more enriched in Tf-positive endosomes with time, whereas pIgA was sorted from these compartments. Live-cell imaging revealed that vesicles containing Fcgamma or pIgA shared similar mobility characteristics and were equivalently affected by depolymerizing microtubules, indicating that both trafficking routes depended to roughly the same extent on intact microtubules. |
Databáze: |
MEDLINE |
Externí odkaz: |
|