Multiplex PCR assay to discriminate four neighbouring species of the Calliptamus genus (Orthoptera: Acrididae) from France.

Autor: Blanchet E; CIRAD Acridologie, Montpellier, F-34398 France. blanchet.elodie@gmail.com, Blondin L, Gagnaire PA, Foucart A, Vassal JM, Lecoq M
Jazyk: angličtina
Zdroj: Bulletin of entomological research [Bull Entomol Res] 2010 Dec; Vol. 100 (6), pp. 701-6. Date of Electronic Publication: 2010 May 27.
DOI: 10.1017/S0007485310000052
Abstrakt: Definition of the genus Calliptamus (Orthoptera: Acrididae) has generated many taxonomic debates. Even now, the existence of different geographical morphs hinders species determination, particularly as concerns females and larvae. Some of these species are observed in southern France and are recognized as potential pests. To circumvent problems of species identification in ecological surveys, we developed a single multiplex PCR method based on mitochondrial Cytochrome Oxydase I diagnostic polymorphisms to differentiate between the four species, Calliptamus italicus, C. wattenwylianus, C. siciliae and C. barbarus, in southern regions of France.
Databáze: MEDLINE