Reduced expression of PDX-1 is associated with decreased beta cell function in chronic pancreatitis.
| Autor: | Mitnala S; Asian Health Care Foundation, Somajiguda, Hyderabad 500082, India. aigres.mit@gmail.com, Pondugala PK, Guduru VR, Rabella P, Thiyyari J, Chivukula S, Boddupalli S, Hardikar AA, Reddy DN |
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| Jazyk: | angličtina |
| Zdroj: | Pancreas [Pancreas] 2010 Aug; Vol. 39 (6), pp. 856-62. |
| DOI: | 10.1097/MPA.0b013e3181d6bc69 |
| Abstrakt: | Objectives: The present study was conducted to monitor the expression of pancreas and duodenal homeobox gene (PDX-1) for assessing beta-cell function in islets from patients with chronic pancreatitis (CP). Methods: Islets isolated from the pancreata of 40 surgical patients categorized as control group, patients with mild CP, and patients with advanced CP were assessed for their yield, size, and glucose-stimulated insulin secretion. Expressions of genes coding for PDX-1, insulin, and glucagon were simultaneously monitored by reverse transcription polymerase chain reaction and confirmed by immunohistochemistry. Results: In comparison with the control group (2673 +/- 592 islet equivalents [IEq]/g), islet yield did not differ much in the patients with mild CP (2344 +/- 738 IEq/g) but was significantly reduced (P < 0.0001) in the patients with advanced CP (731 +/- 167 IEq/g). Although the marginal decrease in islet size observed in the patients with mild CP was not significantly different from that observed in the control group, there was a 58% decrease observed in the patients with advanced CP that was also accompanied by a significant reduction in beta-cell mass (P < 0.05). The expression of insulin and PDX-1 genes, but not of glucagon, was significantly reduced in the patients with advanced CP as confirmed by immunohistochemistry. Islets obtained from the patients with advanced CP retained 53% glucose-stimulated insulin secretion function in comparison with those of the control group. Conclusion: The results indicate that beta-cell dysfunction during progression of CP correlates with the decrease in PDX-1 gene expression. |
| Databáze: | MEDLINE |
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