Autor: |
Buffet-Bataillon S; Service de Bacteriologie, Virologie et Hygiene Hospitaliere, Pole Microorganismes et Hygiene Hospitaliere, CHU de Rennes, France. sylvie.buffet-bataillon@chu-rennes.fr, Bonnaure-Mallet M, de la Pintiere A, Defawe G, Gautier-Lerestif AL, Fauveau S, Minet J |
Jazyk: |
angličtina |
Zdroj: |
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2010 Apr; Vol. 20 (4), pp. 779-81. |
Abstrakt: |
After preliminary tests indicated an increased number of heterotrophic bacteria, we investigated possible sources of contamination in a neonatal intensive care unit (NICU) water distribution system. Scanning electron microscopic examination of flexible metallic hoses associated with the system revealed the presence of a biofilm; partial 16S rDNA sequencing revealed that the biofilm contained Blastomonas natatoria. Purgation of the water system three times a day, reinforced faucet cleaning, decreasing the cold water temperature to 12 degrees , and six repeated chlorinations at concentrations as high as 2 mg/L were not sufficient to eradicate the bacterial contamination. Replacing all of the rubber-interior flexible metallic hoses with teflon-lined hoses followed by heating the water to 70 degrees successfully controlled the bacteria. |
Databáze: |
MEDLINE |
Externí odkaz: |
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