| Autor: |
Cheremnykh ES, Radiukhina NV, Rutkevich PN, Shevelev AIa, Vlasik TN, Voroteliak EA, Vasil'ev AV, Terskikh VV |
| Jazyk: |
ruština |
| Zdroj: |
Tsitologiia [Tsitologiia] 2010; Vol. 52 (3), pp. 219-24. |
| Abstrakt: |
In the present study, human keratinocytes and dermal papilla cells were labeled to investigate their behaviour after intradermal transplantation. Cells were transduced by lentiviral vectors that bore marker gene encoding green fluorescent protein (copGFP) or red fluorescent protein (DsRed). A portion of transgene expressing cells was evaluated by flow cytometry. Genetic constructions that we used provided high level (> 95 %) of transduction of hair follicle cells. In vitro transduced cells were injected under the epidermis of human skin fragments, and these fragments were then transplanted under the skin of immunodeficient mice. Injected epidermal keratinocytes were found, mainly, in hair follicles and partially in a zone of interfollicular epidermis, while dermal papilla cells were found in papilla derma. The results of the present research show that the chosen genetic constructions obtained on a basis of human immunodeficiency lentivirs are capable of effective and stable transduction of human skin cells. Injected cells survived and were found in the corresponding structures of the skin. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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