| Autor: |
Shaw KJ; Department of Chemistry, University of Hull, Cottingham Road, Hull, UKHU6 7RX., Docker PT, Yelland JV, Dyer CE, Greenman J, Greenway GM, Haswell SJ |
| Jazyk: |
angličtina |
| Zdroj: |
Lab on a chip [Lab Chip] 2010 Jul 07; Vol. 10 (13), pp. 1725-8. Date of Electronic Publication: 2010 Apr 23. |
| DOI: |
10.1039/c000357n |
| Abstrakt: |
A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 65 degrees C s(-1) and minimal over- or under-shoot (+/-0.1 degrees C) when reaching target temperatures. In addition, once the required temperature was reached it could be maintained with an accuracy of +/-0.1 degrees C. To demonstrate the functionality of the system, PCR was successfully performed for the amplification of the Amelogenin locus using heating rates and quantities an order of magnitude faster and smaller than current commercial instruments. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
