| Autor: |
Gerashchenko GV; Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv. g.v.gerashchenko@imbg.org.ua, Gordiyuk VV, Skrypkina IY, Kvasha SM, Kolesnik OO, Ugryn DD, Pavlova TV, Zabarovsky ER, Rynditch AV, Kashuba VI |
| Jazyk: |
angličtina |
| Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2009 Jul-Aug; Vol. 81 (4), pp. 81-7. |
| Abstrakt: |
DNA microarray technology comprising NotI-linking clones was used in a large-scale study of genetic and epigenetic changes in colorectal cancer. Analysis of samples from 24 patients revealed methylation, deletions, and amplifications in 137 of 181 NotI clones. For 27 genes/loci, these changes occurred in more than 30% of the tumor samples, suggesting that these genes are involved in the development of colorectal cancer. An analysis of the methylation status of CpG island of the ITGA9 gene/loci by bisulfite sequencing confirmed the NotI microarray data on the gene/loci methylation in colorectal cancer. Aberrations in 19 genes/loci were unknown previously. Their characterization may help ascertain the mechanisms responsible for colorectal cancer development and identify novel diagnostic and prognostic markers. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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