[The influence of pH on cholinesterase hydrolysis of alpha-naphthylacetate in the presence of some cationic detergents].

Autor: Kuznetsova LP, Nikitina ER, Sochilina EE, Vasil'eva KA
Jazyk: ruština
Zdroj: Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2009 Jul-Aug; Vol. 81 (4), pp. 23-7.
Abstrakt: The influence of some cationic detergents on the catalytic activity of the horse blood plasma cholinesterase in reaction of hydrolysis of alpha-naphthylacetate at different pH were investigated. It was shown, that in the absence of detergents in acid pH of the reaction medium the Km value increases, but V remain constant. In the range of pH from 8.5 to 5.0 in the presence of detergents the Km and V values are not practically changed. That is why the activation of cholinesterase hydrolysis of alpha-naphthylacetate in the presence of detergents is considerably higher than that of the neutral pH.
Databáze: MEDLINE