| Autor: |
Wilson J; Police Laboratory, Tulsa Police Department, Tulsa, OK, USA., Fuller V, Benson G, Juroske D, Duvall E, Fu J, Pritchard J, Allen RW |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of forensic sciences [J Forensic Sci] 2010 Jul; Vol. 55 (4), pp. 1050-7. Date of Electronic Publication: 2010 Apr 08. |
| DOI: |
10.1111/j.1556-4029.2010.01371.x |
| Abstrakt: |
A method is described for the quantitation of total human and male DNA. Q-TAT utilizes end-point, multiplex polymerase chain reaction (PCR) amplification of the amelogenin and SRY loci to quantify DNA and incorporates a cloned nonhuman template to detect PCR inhibition. Standard curves of fluorescence from amelogenin or SRY amplicons were generated from amplification of known amounts of NIST traceable SRM-female or SRM-male DNA. Curves showed good linearity up to 500 pg of SRM-template (R(2) > 0.99) and reliably estimated total and male DNA content in casework samples. The nonhuman pRL(null) template included in each PCR was a sensitive indicator of known PCR inhibitors including EDTA, hemin, blue denim dye, and humic acid. Finally, the SRY amplicon was a sensitive indicator of male DNA and, in mixtures, could reliably estimate male DNA present in an excess of female DNA. The Q-TAT multiplex is a reliable quantitation method for forensic DNA typing. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Plný text