PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples.
| Autor: | Medeiros AW; Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil., d'Azevedo P, Pereira RI, Cassenego AP, Van Der Sand S, Frazzon J, Frazzon AP |
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| Jazyk: | angličtina |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2010 Jan-Feb; Vol. 43 (1), pp. 100-1. |
| DOI: | 10.1590/s0037-86822010000100023 |
| Abstrakt: | Introduction: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. Methods: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with HinfI. Only 20 (38.5%) of the 52 strains showed a DNA pattern expected for E. gallinarum and E. casseliflavus. Results: Analysis of the results of this study showed that E. gallinarum and E. casseliflavus are occasionally erroneously identified and confirmed the potential application of 16S rDNA analysis for accurate identification of these species. Conclusions: A correct identification is important to distinguish between intrinsic and acquired vancomycin resistance. |
| Databáze: | MEDLINE |
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