| Autor: |
van Buul JD; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands. j.vanbuul@sanquin.nl, van Rijssel J, van Alphen FP, van Stalborch AM, Mul EP, Hordijk PL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of biomedicine & biotechnology [J Biomed Biotechnol] 2010; Vol. 2010, pp. 120328. Date of Electronic Publication: 2010 Mar 15. |
| DOI: |
10.1155/2010/120328 |
| Abstrakt: |
In the initial stages of transendothelial migration, leukocytes use the endothelial integrin ligands ICAM-1 and VCAM-1 for strong adhesion. Upon adhesion of the leukocyte to endothelial ICAM-1, ICAM-1 is clustered and recruited to the adhered leukocyte, promoting strong adhesion. In this study, we provide evidence for the colocalization of VCAM-1 at sites of ICAM-1 clustering. Anti-ICAM-1 antibody-coated beads were used to selectively cluster and recruit ICAM-1 on primary human endothelial cells. In time, co-localization of ICAM-1 and VCAM-1 around the adherent beads was observed. Biochemical pull-down assays showed that ICAM-1 clustering induced its association to VCAM-1, suggesting a physical link between these two adhesion molecules. The association was partly dependent on lipid rafts as well as on F-actin and promoted adhesion. These data show that VCAM-1 can be recruited, in an integrin-independent fashion, to clustered ICAM-1 which may serve to promote ICAM-1-mediated leukocyte adhesion. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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