[Enzyme immunoassay-based analysis of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia].

Autor:	Karavaev VS, Riabchenko AV, Beklemishev AB
Jazyk:	ruština
Zdroj:	Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2010 Jan-Feb (1), pp. 20-3.
Abstrakt:	Aim: To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB). Materials and Methods: Recombinant antigens OspC B. garinii and OspC B. afrelii, serum samples from patients with TBB were used as well as solid-phase enzyme immunoassay and parametric and non-parametric statistical methods. Results: Higher antigenic activity of B. garinii OspC compared with OspC from B. afzelii was observed when these recombinant proteins were compared in enzyme immunoassay. Detection rate of class M and G immunoglobulins to B. garinii OspC in sera of patients with TBB was 60.5% and 70% respectively. Conclusion: Obtained results indicate high immunoreactivity of OspC recombinant proteins from B. garinii and B. afzelii and point to perspective of their combined use for serological diagnostics of TBB.
Databáze:	MEDLINE
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