[Rapid identification methods of yeasts during fungaemia].

Autor: Badenhorst L; Departement Mediese Mikrobiologie, Universiteit van die Oranje-Vrystaat, Bloemfontein., Botha PL, van Rensburg MN
Jazyk: afrikánština
Zdroj: South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde [S Afr Med J] 1991 Mar 16; Vol. 79 (6), pp. 304-6.
Abstrakt: A wet preparation method, modified serum test and a modified disc diffusion method were evaluated in order to identify yeasts more rapidly during fungaemia. A total of 2,932 blood cultures were processed, of which 54 (1.8%) yielded yeasts. By using these methods, Candida albicans was identified within 3 hours, after yeast growth in the blood culture was confirmed by Gram stain. The wet preparation examinations were accurate in 60% of cases and the serum test in 95% of cases. After growth was detected, yeast species other than C. albicans were identified within 24 hours using the modified disc diffusion method. C. albicans (50%), C. tropicalis (22%) and C. parapsilosis (22%) were the species most frequently isolated. Fungaemia occurred most often after antimicrobial therapy and abdominal conditions.
Databáze: MEDLINE