Routine using pattern and performance of diagnostic tests for tuberculosis on a university hospital.
| Autor: | Dundar D; Department of Microbiology and Clinical Microbiology, Medical Faculty, Kocaeli University, 41380 Kocaeli, Turkey., Sayan M, Arslan Z, Tamer GS, Dundar V |
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| Jazyk: | angličtina |
| Zdroj: | The American journal of the medical sciences [Am J Med Sci] 2010 Mar; Vol. 339 (3), pp. 244-8. |
| DOI: | 10.1097/MAJ.0b013e3181cbfe40 |
| Abstrakt: | Introduction: Nucleic acid amplification tests to detect Mycobacterium tuberculosis in clinical specimens are used increasingly as a laboratory tool. We aimed to investigate the routine using pattern and the effects on therapeutic decision of diagnostic tests for tuberculosis in our hospital. Methods: In this descriptive study, we investigated retrospectively the routine using pattern and the effects on therapeutic decision of diagnostic tests for tuberculosis. Patients with discordant results were clinically evaluated retrospectively by a chest physician. Samples were tested for the presence of M. tuberculosis by a smear technique, M. tuberculosis culture growth technique (Löwenstein-Jensen and/or BACTEC-960), and IS6110 polymerase chain reaction (PCR). Results: Culture positivity was 7.2% (83 of 1159 patients). In total, 198 (62.4%) were tested with PCR, acid-fast bacilli, and culture. On the basis of culture results as a gold standard, sensitivity, specificity, positive predictive value, and negative predictive value of PCR were 46%, 89%, 23%, and 93.5%, respectively. Conclusions: Selection of appropriate patients for further testing and exclusion of low-risk patients from microbiologic testing by experienced clinicians may help to optimize the positive predictive value of PCR. |
| Databáze: | MEDLINE |
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