Enhancement on the Europium emission band of Europium chlortetracycline complex in the presence of LDL.

Autor: Teixeira Ldos S; Centro de Lasers e Aplicações, IPEN/CNEN-SP, Brazil., Grasso AN, Monteiro AM, Neto AM, Vieira ND Jr, Gidlund M, Courrol LC
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2010 May 01; Vol. 400 (1), pp. 19-24. Date of Electronic Publication: 2010 Feb 01.
DOI: 10.1016/j.ab.2010.01.030
Abstrakt: Low-density lipoprotein (LDL) particles are the major cholesterol-carrying lipoprotein in the human circulation from the liver to peripheral tissues. High levels of LDL-Cholesterol (LDL-C) are known risk factor for the development of coronary artery disease (CAD). The most common approach to determine the LDL-C in the clinical laboratory involves the Friedewald formula. However, in certain situations, this approach is inadequate. In this paper we report on the enhancement on the Europium emission band of Europium chlortetracycline complex (CTEu) in the presence of LDL. The emission intensity at 615 nm of the CTEu increases with increasing amounts of LDL. This phenomenon allowed us to propose a method to determine the LDL concentration in a sample composed by an aqueous solution of LDL. With this result we obtained LDL calibration curve, LOD (limit of detection) of 0.49 mg/mL and SD (standard deviation) of 0.003. We observed that CTEu complex provides a wider dynamic concentration-range for LDL determination than that from Eu-tetracycline previously. The averaged emission lifetimes of the CTEu and CTEu with LDL (1.5 mg/mL) complexes were measured as 15 and 46 micros, respectively. Study with some metallic interferents is presented.
(2010 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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