[Preparations of the specific antibodies against exon 2 and exon 3 of human tau protein].

Autor:	Chen C; College of Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China., Wang GR, Shi Q, Zhang BY, Mei GY, Li Y, Wang X, Zhou RM, Han J, Zhao YJ, Dong XP
Jazyk:	čínština
Zdroj:	Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi] 2009 Apr; Vol. 23 (2), pp. 146-8.
Abstrakt:	Objective: To prepare the specific antibodies against exon 2 and exon 3 of human tau protein. Methods: Sequences encoding exon 2 and exon 3 of human tau protein were amplified from human peripheral blood DNA and cloned into a prokaryotic expression vector pGEX-2T. Fusion proteins GST-E2 and GST-E3 were expressed and purified from E. coli system. The antisera were elicited by immunization of the purified fusion proteins to rabbits and mice. The specific antibodies were purified by Protein G/A and CNBr-activated sepharose 4B coupled with GST protein. The specificity and sensitivity of the purified antibodies were evaluated by Western blotting and ELISA. Results: Recombinant fusion proteins GST-E2 and GST-E3 were expressed and purified from E. coli, which showed Mr. 29 x 10(3). Various antisera were collected from the immunized experimental animals. Reliable immunoreactive specificity and titers of the purified antibodies against exon 2 and exon 3 of human tau protein were confirmed by Western blotting and ELISA after serial purification processes. Conclusion: Four specific antibodies against exon 2 and exon 3 of human tau protein have been successfully prepared, which provides basis for analyzing the role of tau in neurodegenerative diseases.
Databáze:	MEDLINE
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