[Influence of immunodepressors on population level and adhesive characteristics of opportunistic bacteria in large intestine of white mice].

Autor:	Shitov LN, Romanov VA
Jazyk:	ruština
Zdroj:	Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2009 Nov-Dec (6), pp. 12-6.
Abstrakt:	Aim: To assess the effects of cyclophosphamide (CP), methotrexate (MT), and prednisolone (PS) on the levels of staphylococci, enterococci, Escherichia coli (EC) and lactose-negative enterobacteria (LNEB) in feces of white mice as well as to determine adhesive properties of isolated microorganisms. Materials and Methods: Drugs were administered intragastrically during 6 weeks; CP in dose 35.4 mg/kg daily; MT - 2.5 mg/kg over week; PS - 2.5 mg/kg daily; sterile distilled water was administered to controls. Microflora was assessed by common methods; adhesion of microorganisms to group 0 (I) human erythrocytes was determined. Results: Administration of prednisolone leads to increase of isolation rate of staphylococci, CP increased levels of LNEB and EC with suppressed enzymatic activity (P < 0.05). MT decreased isolation rate of typical EC (P < 0.05). MT also decreased degree of adhesion of EC and LNEB, whereas PS enhanced adhesiveness of LNEB, and CP decreased adhesion of staphylococci (P < 0.05). Statistically significant correlation between number and adhesiveness of enterobacteria was established (r = 0.73, P < 0.01). Conclusion: Immunodepressors from different groups have non-similar effects on quantitative characteristics of microflora and adhesiveness of isolated opportunistic microorganisms.
Databáze:	MEDLINE
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