| Abstrakt: |
The cultivation of Silene vulgaris (M.) G. callus culture on the nutrient mediums contained carbohydrates, phytohormones, nitrogen, and phosphate has led to the modification of the arabinogalactane structure from the cell walls. It was noticed that a sucrose concentration increase in the cultivation medium led to an increase of the arabinogalactane enzyme yield with a molecular weight more than 300 kDa and a decrease of the yield of fragments with molecular weight less than 300 kDa. The sucrose concentration increase in the nutrient medium entailed the increase of arabinose and galactose content in the fragment with the molecular weight more than 300 kDa and a decrease in the fragment with a molecular weight of 100-300 kDa. On the nutrient medium containing a mix of sucrose and arabinose, the yield of the fraction with a molecular weight more than 300 kDa and the amount of arabinose residues increased, and the yield of minor fragments and the content of arabinose and galactose residues, included in these, decreased. On the medium containing an increased concentration of 2,4-dichlorphenoxiacetic acid, the yield of high-molecular fragment and the concentration of arabinose are two times increased. The decreasing of the amount of arabinose and galactose residues in the fragment with a molecular weight of 300 kDa was observed at a lack of nitrogen or phosphate in the nutrient medium. |
