| Autor: |
Carvalho GD; Faculdade de Ciências Biológicas, Universidade Federal de Viçosa, da Saúde, UNIVIÇOSA, Viçosa, MG, Brazil., Peconick AP, Medeiros CL, Vargas-V MI, Patarroyo JH |
| Jazyk: |
portugalština |
| Zdroj: |
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria [Rev Bras Parasitol Vet] 2008 Sep; Vol. 17 Suppl 1, pp. 9-13. |
| Abstrakt: |
The mucosal immunization consists on antigen administration in these surfaces it is a not invasive method, inductor of local and systemic immune response. This work evaluated the immune response of the synthetic vaccine anti-tick Rhipicephalus (Boophilus) microplus SBm7462®, via oral and nasal routes. Were used 60 BALB/c mice, divided in three groups of 20 animals each (I: oral immunization; II: nasal immunization; III: animals not immunized). Nine and 15 days after the first and the second immunization was collected, separately for each group, T cells from the immunized animals, cultivating them per 10 days. After the incubation, were determined the percentage of cellular viability and the specific nature of this T cells, which had held as memory T cell. The averages of T cell SBm7462-reagents had been submitted to the analysis of variance and comparison for Tukey test, 5% of probability. Group II presented higher cellular proliferation "in vitro". For ELISA test, were positive only two animals in group I and eight in group II. The oral and nasal routs alternatively viable for immunization with the synthetic peptide SBm7462®, however require greater number of doses to induce responses with high levels of immunoglobulins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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