Autor: |
Fulton RW; Department of Veterinary Pathobiology, Oklahoma State University, Center for Veterinary Health Sciences, Stillwater, Oklahoma, USA. robert.fulton@okstate.edu, Whitley EM, Johnson BJ, Ridpath JF, Kapil S, Burge LJ, Cook BJ, Confer AW |
Jazyk: |
angličtina |
Zdroj: |
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire [Can J Vet Res] 2009 Oct; Vol. 73 (4), pp. 283-91. |
Abstrakt: |
The prevalence of bovine viral diarrhea virus (BVDV) in persistently infected (PI) cattle in beef breeding herds was determined using 30 herds with 4530 calves. The samples were collected by ear notches and tested for BVDV antigens using immunohistochemistry (IHC) and antigen capture enzyme-linked immunosorbent assay (ACE). Animals with initial positives on both IHC and ACE were sampled again using both tests and serums were collected for viral propagation and sequencing of a viral genomic region, 5'-untranslated region (5'-UTR) for viral subtyping. Samples were also collected from the dams of PI calves. There were 25 PI calves from 4530 samples (0.55%) and these PI calves were from 5 of the 30 herds (16.7%). Two herds had multiple PI calves and 3 herds had only 1 PI calf. Only 1 of the 25 dams with a PI calf was also PI (4.0%). The subtype of all the PI isolates was BVDV1b. Histories of the ranches indicated 23 out of 30 had herd additions of untested breeding females. Twenty-four of the 30 herds had adult cowherd vaccinations against BVDV, primarily using killed BVDV vaccines at pregnancy examination. |
Databáze: |
MEDLINE |
Externí odkaz: |
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