The rate and molecular spectrum of spontaneous mutations in Arabidopsis thaliana.

A:T transitions. We explain this very biased spectrum of base substitution mutations as a result of two main processes: deamination of methylated cytosines and ultraviolet light-induced mutagenesis. -->
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Grant Information: R01 GM036827 United States GM NIGMS NIH HHS; GM36827 United States GM NIGMS NIH HHS
Substance Nomenclature: 0 (DNA, Intergenic)
0 (DNA, Plant)
8J337D1HZY (Cytosine)
Entry Date(s): Date Created: 20100102 Date Completed: 20100125 Latest Revision: 20240519
Update Code: 20240519
PubMed Central ID: PMC3878865
DOI: 10.1126/science.1180677
PMID: 20044577
Autor: Ossowski S; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany., Schneeberger K, Lucas-Lledó JI, Warthmann N, Clark RM, Shaw RG, Weigel D, Lynch M
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2010 Jan 01; Vol. 327 (5961), pp. 92-4.
DOI: 10.1126/science.1180677
Abstrakt: To take complete advantage of information on within-species polymorphism and divergence from close relatives, one needs to know the rate and the molecular spectrum of spontaneous mutations. To this end, we have searched for de novo spontaneous mutations in the complete nuclear genomes of five Arabidopsis thaliana mutation accumulation lines that had been maintained by single-seed descent for 30 generations. We identified and validated 99 base substitutions and 17 small and large insertions and deletions. Our results imply a spontaneous mutation rate of 7 x 10(-9) base substitutions per site per generation, the majority of which are G:C-->A:T transitions. We explain this very biased spectrum of base substitution mutations as a result of two main processes: deamination of methylated cytosines and ultraviolet light-induced mutagenesis.
Databáze: MEDLINE
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