| Autor: |
Reller ME; Division of Medical Microbiology, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA., Alcabasa RC, Lema CA, Carroll KC |
| Jazyk: |
angličtina |
| Zdroj: |
American journal of clinical pathology [Am J Clin Pathol] 2010 Jan; Vol. 133 (1), pp. 107-9. |
| DOI: |
10.1309/AJCPO3QWOU8CYGEU |
| Abstrakt: |
We compared 3 rapid assays for Clostridium difficile with a cell culture cytotoxicity neutralization assay (CCNA). Of 600 stool samples, 46 were positive for toxigenic C difficile. Both rapid common antigen assays were highly sensitive (91.3%-100%) and, therefore, were appropriate screening tests. The rapid toxin assay had poor sensitivity (61%) but excellent specificity (99.3%). Testing stools for glutamate dehydrogenase (step 1) and those positive with a rapid toxin assay (step 2) would correctly classify 81% of submitted specimens within 2 hours, including during periods of limited staffing (evenings, nights, and weekends). CCNA could then be used as a third step to test rapid toxin-negative samples, thereby providing a final result for the remaining 19% of samples by 48 to 72 hours. The use of rapid assays as outlined could enhance timely diagnosis of C difficile. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
