| Autor: |
Lisovyy OO; Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, Kiev, Ukraine., Dosenko VE, Nagibin VS, Tumanovska LV, Korol MO, Surova OV, Moibenko OO |
| Jazyk: |
angličtina |
| Zdroj: |
Acta biochimica Polonica [Acta Biochim Pol] 2009; Vol. 56 (4), pp. 687-94. Date of Electronic Publication: 2009 Dec 11. |
| Abstrakt: |
It is well known that 5-lipoxygenase derivates of arachidonic acid play an important pathogenic role during myocardial infarction. Therefore, the gene encoding arachidonate 5-lipoxygenase (ALOX5) appears to be an attractive target for RNA interference (RNAi) application. In experiments on cultivated cardiomyocytes with anoxia-reoxygenation (AR) and in vivo using rat model of heart ischemia-reperfusion (IR) we determined influence of ALOX5 silencing on myocardial cell death. ALOX5 silencing was quantified using real-time PCR, semi-quantitative PCR, and evaluation of LTC(4) concentration in cardiac tissue. A 4.7-fold decrease of ALOX5 expression (P < 0.05) was observed in isolated cardiomyocytes together with a reduced number of necrotic cardiomyocytes (P < 0.05), increased number live (P < 0.05) and unchanged number of apoptotic cells during AR of cardiomyocytes. Downregulation of ALOX5 expression in myocardial tissue by 19% (P < 0.05) resulted in a 3.8-fold reduction of infarct size in an open chest rat model of heart IR (P < 0.05). Thus, RNAi targeting of ALOX5 protects heart cells against IR injury both in culture and in vivo. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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