| Autor: |
Lee A; Office of Biotechnology Products, U.S. Food and Drug Administration, Silver Spring, MD 20903, USA., McVey J, Faustino P, Lute S, Sweeney N, Pawar V, Khan M, Brorson K, Hussong D |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 2010 Feb; Vol. 76 (3), pp. 695-700. Date of Electronic Publication: 2009 Dec 04. |
| DOI: |
10.1128/AEM.01825-09 |
| Abstrakt: |
Filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. Using Hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. We show that consistent H. pseudoflava passage occurs through 0.2-microm-rated filters. This is in contrast to an absence of significant passage of nutritionally challenged bacteria that are of similar size (i.e., hydrodynamic diameter) but dissimilar geometry. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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