| Autor: |
Fini JB; UMR CNRS 7221, Evolution des Regulations Endocriniennes, Departement Regulations, Developpement et Diversite Moleculaire, Museum National d'Histoire Naturelle, 75231 Paris, France., Pallud-Mothré S, Le Mével S, Palmier K, Havens CM, Le Brun M, Mataix V, Lemkine GF, Demeneix BA, Turque N, Johnson PE |
| Jazyk: |
angličtina |
| Zdroj: |
Environmental science & technology [Environ Sci Technol] 2009 Dec 01; Vol. 43 (23), pp. 8895-900. |
| DOI: |
10.1021/es9008954 |
| Abstrakt: |
While numerous detection methods exist for environmental heavy metal monitoring, easy-to-use technologies combining rapidity with in vivo measurements are lacking. Multiwell systems exploiting transgenic tadpoles are ideal but require time-consuming placement of individuals in wells. We developed a real-time flow-through system, based on Fountain Flow cytometry, which measures in situ contaminant-induced fluorescence in transgenic amphibian larvae immersed in water samples. The system maintains the advantages of transgenic amphibians, but requires minimal human intervention. Portable and self-contained, it allows on-site measurements. Optimization exploited a transgenic Xenopus laevis bearing a chimeric gene with metal responsive elements fused to eGFP. The transgene was selectively induced by 1 microM Zn(2+). Using this tadpole we show the continuous flow method to be as rapid and sensitive as image analysis. Flow-through readings thus accelerate the overall process of data acquisition and render fluorescent monitoring of tadpoles suitable for on-site tracking of heavy metal pollution. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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