| Autor: |
Rivas-Castillo AM; Division of Natural and Exact Sciences, Department of Biology, University of Guanajuato, Noria Alta S/N, P.O. Box. 187, Guanajuato, 36050, Gto, México., Yasbin RE, Robleto E, Nicholson WL, Pedraza-Reyes M |
| Jazyk: |
angličtina |
| Zdroj: |
Current microbiology [Curr Microbiol] 2010 Apr; Vol. 60 (4), pp. 263-7. Date of Electronic Publication: 2009 Nov 19. |
| DOI: |
10.1007/s00284-009-9535-3 |
| Abstrakt: |
The role played by the Y-family DNA polymerases YqjH and YqjW in protecting sporulating cells of Bacillus subtilis from DNA damage was determined. The absence of either yqjH and/or yqjW not only reduced sporulation efficiency but also sensitized the sporulating cells to hydrogen peroxide, tert-butylhydroperoxide (t-BHP), mitomycin-C (M-C), and UV-C radiation. Moreover, these DNA-damaging agents increased the mutation frequency of wild-type sporulating cells to 4-azaleucine, but the production of mutants was YqjH- and YqjW-dependent. In conclusion, the results presented here indicate that YqjH/YqjW-dependent-translesion synthesis (TLS) operates in sporulating B. subtilis cells and contributes in processing spontaneous and artificially induced genetic damage, which is apparently required for an efficient sporulation process. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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