| Autor: |
Klein CB; Institute of Environmental Medicine, New York University Medical Center, NY 10016., Conway K, Wang XW, Bhamra RK, Lin XH, Cohen MD, Annab L, Barrett JC, Costa M |
| Jazyk: |
angličtina |
| Zdroj: |
Science (New York, N.Y.) [Science] 1991 Feb 15; Vol. 251 (4995), pp. 796-9. |
| DOI: |
10.1126/science.1990442 |
| Abstrakt: |
Transfer of a normal Chinese hamster X chromosome (carried in a mouse A9 donor cell line) to a nickel-transformed Chinese hamster cell line with an Xq chromosome deletion resulted in senescense of these previously immortal cells. At early passages of the A9/CX donor cells, the hamster X chromosome was highly active, inducing senescence in 100% of the colonies obtained after its transfer into the nickel-transformed cells. However, senescence was reduced to 50% when Chinese hamster X chromosomes were transferred from later passage A9 cells. Full senescing activity of the intact hamster X chromosome was restored by treatment of the donor mouse cells with 5-azacytidine, which induced demethylation of DNA. These results suggest that a senescence gene or genes, which may be located on the Chinese hamster X chromosome, can be regulated by DNA methylation, and that escape from senescence and possibly loss of tumor suppressor gene activity can occur by epigenetic mechanisms. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
