| Autor: |
Shaw KJ; Department of Chemistry, University of Hull, Cottingham Road, Hull, UK HU6 7RX., Joyce DA, Docker PT, Dyer CE, Greenman J, Greenway GM, Haswell SJ |
| Jazyk: |
angličtina |
| Zdroj: |
Lab on a chip [Lab Chip] 2009 Dec 07; Vol. 9 (23), pp. 3430-2. Date of Electronic Publication: 2009 Sep 21. |
| DOI: |
10.1039/b913309g |
| Abstrakt: |
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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