| Autor: |
Kaberniuk AA, Labyntsev AIu, Kolybo DV, Oliĭnyk OS, Redchuk TA, Korotkevych NV, Horchev VF, Karakhim SO, Komisarenko SV |
| Jazyk: |
ukrajinština |
| Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2009 Jan-Feb; Vol. 81 (1), pp. 67-77. |
| Abstrakt: |
Diphtheria toxin's B subunit provides toxin interaction with its receptor on the cell surface and translocation of toxin's A subunit from endosome to cytozole of sensitive cells. Functional analogues of B subunit with fluorescent label are considered as perspective tools for studying the above mentioned processes. The aim of the work was to obtain fluorescent B subunit analogues and to detect the specificity of their interaction with Vero line cells. B subunit fluorescent analogues were obtained in two different ways. The first one was B subunit chemical conjugation with fluorescein isothiocyanate and the second one was genetic fusion of recombinant B subunit chain with enhanced green fluorescent protein chain. Specific interaction of B subunit fluorescent derivatives with Vero cells was studied by flow cytometry and confocal microscopy. Using competitive analysis it was shown that B subunit fluorescent analogues possessed different affinity for cells. The affinity of EGFP-SbB was higher than FITC-SbB. Our results indicate the possibility to use the fluorescent derivatives of B subunit as tools for identification of diphtheria toxin's receptor (HB-EGF) expression on the cell surface as well as for studying the interaction and penetration of diphtheria toxin to the cell. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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