| Autor: |
Krasnikova MS; Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Timiryazevskaya 42, 127550 Moscow, Russia., Milyutina IA, Bobrova VK, Ozerova LV, Troitsky AV, Solovyev AG, Morozov SY |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of biomedicine & biotechnology [J Biomed Biotechnol] 2009; Vol. 2009, pp. 952304. Date of Electronic Publication: 2009 Oct 13. |
| DOI: |
10.1155/2009/952304 |
| Abstrakt: |
TAS loci in plant genomes encode transacting small interfering RNAs (ta-siRNAs) that regulate expression of a number of genes. The function of TAS3 precursor in Arabidopsis thaliana is controlled by two miR390 target sites flanking two ta-siARF sequences targeting mRNAs of ARF transcription factors. Cleavage of the 3'-miR390-site initiates ta-siRNAs biogenesis. Here we describe the new method for identification of plant ta-siRNA precursors based on PCR with oligodeoxyribonucleotide primers mimicking miR390. The method was found to be efficient for dicotiledonous plants, cycads, and mosses. Based on sequences of amplified loci and a database analysis, a novel type of miR390-dependent TAS sequences was identified in dicots. These TAS loci are characterized by a smaller distance between miR390 sites compared to TAS3, a single copy of ta-siARF, and a sequence conservation pattern pointing to the possibility that processing of novel TAS-like locus is initiated by cleavage of the 5'-terminal miR390 target site. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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