Angiotensin converting enzyme DD genotype is associated with development of rheumatic heart disease in Egyptian children.

Autor: Morsy MM; Department of Pediatrics and Pediatric Cardiology, Sohag Faculty of Medicine, Sohag University Hospital, Sohag, Egypt. mofeedf@yahoo.com, Abdelaziz NA, Boghdady AM, Ahmed H, Abu Elfadl EM, Ismail MA
Jazyk: angličtina
Zdroj: Rheumatology international [Rheumatol Int] 2011 Jan; Vol. 31 (1), pp. 17-21. Date of Electronic Publication: 2009 Oct 22.
DOI: 10.1007/s00296-009-1208-9
Abstrakt: Angiotensin converting enzyme (ACE) gene polymorphism was previously studied in some cardiovascular diseases. There are only few studies which investigated this polymorphism in patients with rheumatic heart disease (RHD). The results of these investigations are inconsistent. Furthermore, gene polymorphism distribution is different in various ethnic populations. We conducted this study to demonstrate this gene polymorphism in Egyptian children with RHD. Leukocytes DNA was extracted from 139 patients with RHD and 79 healthy control children. After amplification by the PCR, the products were separated by electrophoresis in 6% polyacrylamide gel and visualized after ethidium bromide staining with UV light. The PCR product is a 190-bp fragment in the absence of the insertion (D allele) and a 490-bp fragment in the presence of the insertion (I allele). Gene polymorphism was as follows: DD gene when lane contains only 190-bp fragment, II gene when lane contains only 490-bp fragment and ID gene when lane contains both fragments. We found that gene polymorphism in both control and patients groups followed the following order of distribution from highest to lowest: ID, II, DD gene. The frequency in control group was 49.4, 36.7, and 13.9%, respectively. In patients groups, the gene frequency was 42.5, 30.9, and 26.6%, respectively. DD gene frequency differs significantly between the two groups. We concluded that patients with RHD have a higher ACE-DD genotype than normal control. ACE-DD genotype may be a risk factor for RHD in Egyptian children.
Databáze: MEDLINE
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