| Autor: |
Lavrent'eva EV, Shagzhina AP, Babasanova OB, Dunaevskiĭ IaE, Namsaraev ZB, Barkhutova DD |
| Jazyk: |
ruština |
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2009 Sep-Oct; Vol. 45 (5), pp. 538-43. |
| Abstrakt: |
Two strains of alkaliphilic thermophile bacteria of the genus Anoxybacillus from hydrothermal vents of Lake Baikal were detected and characterized. It was demonstrated that proteinases secreted by these bacteria had wide substrate specificity, hydrolyzed proteins and n-nitroanilide substrates, and showed maximal activity at pyroglutamyl-alanine-alanine-leucine n-nitroanilide hydrolysis. We determined maximal activity of the proteinases at alkaline pH values (10.0-10.5), the enzymes were thermostable and were characterized by a wide thermal optimum (55-70 degrees C). The results of inhibitor analysis and substrate specifity examination of extracellular enzymes demonstrated their belonging to the subtilisin-like serine proteinases. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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