Expression of the Arabidopsis thaliana histone gene AtHTA1 enhances rice transformation efficiency.

Autor: Zheng YE; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China., He XW; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China., Ying YH; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China., Lu JF; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China., Gelvin SB; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA., Shou HX; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China. Electronic address: huixia@zju.edu.cn.
Jazyk: angličtina
Zdroj: Molecular plant [Mol Plant] 2009 Jul; Vol. 2 (4), pp. 832-837. Date of Electronic Publication: 2009 Jun 15.
DOI: 10.1093/mp/ssp038
Abstrakt: We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA1 either transiently or in stably transformed cells improved rice transformation efficiency.
Databáze: MEDLINE
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