Vascular endothelial growth factor production is regulated by gene polymorphisms.
| Autor: | Mohammadi M; Department of Immunology, Kerman University of Medical Sciences, Iran. mo_mohammadi@kmu.ac.ir, Bazrafshani MR, Day PJ, Ollier WE |
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| Jazyk: | angličtina |
| Zdroj: | Iranian journal of immunology : IJI [Iran J Immunol] 2009 Sep; Vol. 6 (3), pp. 119-29. |
| DOI: | IJIv6i3A2 |
| Abstrakt: | Background: Vascular endothelial growth factor (VEGF) has a key role in angiogenesis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region. Objectives: In this study, the association between the combination of VEGF -1154 G and -2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship between VEGF polymorphisms and the influence of TNF- and IL-4 on VEGF production was studied. Methods: VEGF -1154 G/A and -2578 C/A were detected using ARMS-PCR. To determine the impact of combinations of these two polymorphisms on VEGF production; PBMCs were stimulated by LPS and VEGF production was measured by ELISA. Results: The combinations of -1154 GG/-2578 CC and -1154 GG/-2578 CA were significantly associated with higher VEGF production (p<0.0001). Production of VEGF was significantly influenced by TNF- in individuals who had certain VEGF genotype combinations. Although VEGF production was dramatically suppressed by IL-4, it was not dependent on VEGF genotype. Conclusions: Since TNF- has influence on the graft outcome, to avoid allocation of grafts from high TNF- producer donors to recipients, it might be useful to predict and minimize graft rejection by having prior knowledge of TNF- and also VEGF genotypes especially -1154 G/A and -2578 C/A VEGF. |
| Databáze: | MEDLINE |
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