Virtual-core flow cytometry.
| Autor: | Swalwell JE; Department of Oceanography, University of Washington, Seattle, Washington 98105, USA., Petersen TW, van den Engh G |
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| Jazyk: | angličtina |
| Zdroj: | Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2009 Nov; Vol. 75 (11), pp. 960-5. |
| DOI: | 10.1002/cyto.a.20792 |
| Abstrakt: | Traditional flow cytometers use a sheath fluid to position particles or cells for cytometric measurements, but the need for sheath fluid greatly complicates flow cytometric instrumentation. A cytometric detector that is free of the requirements of sheath fluid can simplify the design of flow cytometers and can extend their use into a number of areas. We designed a flow cytometer that uses a combination of three photodetectors to sense the position of a particle in sample stream. The position-sensitive detectors create a virtual core in the sample stream that eliminates the need for sheath fluid. In this article, we demonstrate the efficacy of a virtual-core flow cytometer (VCFC) using test particles, immunofluorescently labeled thymocytes, and raw seawater. The VCFC performs accurate measurements that can be used for a number of uses including environmental monitoring or simple immunology tests. (Copyright 2009 International Society for Advancement of Cytometry.) |
| Databáze: | MEDLINE |
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