| Autor: |
Ottink OM; Institute for Molecules and Materials, Department of Biophysical Chemistry, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands., Nelissen FH, Derks Y, Wijmenga SS, Heus HA |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 2010 Jan 15; Vol. 396 (2), pp. 280-3. Date of Electronic Publication: 2009 Sep 11. |
| DOI: |
10.1016/j.ab.2009.09.013 |
| Abstrakt: |
S-Adenosyl-L-methionine (SAM) is the preferred cofactor for biological methyl group transfers to various substrates such as nucleic acids, proteins, and lipids. Here we present stereospecific (>95% of the desired enantiomer) and high-yield preparation of four fluorescent and biologically active SAM analogs and demonstrate their usefulness in binding studies. Using a fluorescence titration experiment, we obtained a K(d) of 0.38 microM for the S-2,6-diaminopurinylmethionine-SAM-III riboswitch complex. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full Text from ScienceDirect