High-performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats.

Autor: Bertram RM; Department of Pharmaceutical Sciences and Pharmacology, Washington State University, Pullman, 99164-6534, USA., Takemoto JK, Remsberg CM, Vega-Villa KR, Sablani S, Davies NM
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2010 May; Vol. 24 (5), pp. 516-21.
DOI: 10.1002/bmc.1320
Abstrakt: A high-performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna C(18) column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.04 v/v) with a flow rate of 0.6 mL/min. The calibration curves were linear over the range of 0.5-100.0 microg/mL. The mean extraction efficiency was between 98.9 and 109%. The precision of the assay was 0.069-18.4% (RSD%), and within 20% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <15% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and to nutraceutical product analysis.
(2009 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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