Preparation of a new chromogenic substrate to assay for beta-galactanases that hydrolyse type II arabino-3,6-galactans.

3)- and/or beta-(1-->6)-d-galactanases. It will enable routine large-scale screening of beta-galactanases from crude enzyme preparations and microorganism cultures, and is suitable for profiling activity during purification processes. -->
Substance Nomenclature: 0 (Chromogenic Compounds)
0 (Galactans)
EC 3.2.1.- (Glycoside Hydrolases)
EC 3.2.1.- (beta-galactanase)
Entry Date(s): Date Created: 20090901 Date Completed: 20091214 Latest Revision: 20090925
Update Code: 20240829
DOI: 10.1016/j.carres.2009.07.014
PMID: 19717142
Autor: Ling NX; University of Melbourne, Victoria, Australia., Pettolino F, Liao ML, Bacic A
Jazyk: angličtina
Zdroj: Carbohydrate research [Carbohydr Res] 2009 Oct 12; Vol. 344 (15), pp. 1941-6. Date of Electronic Publication: 2009 Aug 12.
DOI: 10.1016/j.carres.2009.07.014
Abstrakt: A chromogenic assay using RB5-dGA, Reactive Black 5 (RB5) dye covalently coupled to de-arabinosylated gum arabic (dGA), was developed for rapid screening of beta-galactanases. dGA was prepared by partial acid hydrolysis (0.25M trifluoroacetic acid for 2h at 90-95 degrees C) of gum Arabic (GA) from Acacia senegal. The dGA exhibited a median molecular mass of approximately 10kDa, corresponding to a degree of polymerisation (DP) approximately 60. It was devoid of Ara residues, and contained mostly Galp (68mol%) together with GlcpA (30mol%). The Galp residues were (1,6)- (34mol%), (1,3)- (3mol%) and (1,3,6)- (26mol%) linked, and the GlcAp residues were primarily terminal (28mol%) together with a small amount of (1,4)-linked (2mol%), as expected for a type II (3,6)-galactan. The new chromogenic assay is simple, cost effective, relatively sensitive, and is specific for either beta-(1-->3)- and/or beta-(1-->6)-d-galactanases. It will enable routine large-scale screening of beta-galactanases from crude enzyme preparations and microorganism cultures, and is suitable for profiling activity during purification processes.
Databáze: MEDLINE
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