| Autor: |
Pontier DB; Hubrecht Institute-Koninklijke Nederlandse Akademie van Wetenschappen, University Medical Centre Utrecht, Utrecht, The Netherlands., Kruisselbrink E, Guryev V, Tijsterman M |
| Jazyk: |
angličtina |
| Zdroj: |
Nature methods [Nat Methods] 2009 Sep; Vol. 6 (9), pp. 655-7. Date of Electronic Publication: 2009 Aug 16. |
| DOI: |
10.1038/nmeth.1362 |
| Abstrakt: |
Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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