A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction.

Autor: Couto MS; Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza, Ceará CEP: 60.425-540, Brazil., Cordeiro Rde A, Rocha MF, Grangeiro TB, Leitão Junior NP, Bandeira Tde J, Sidrim JJ, Brilhante RS
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2009 Sep; Vol. 65 (1), pp. 73-5.
DOI: 10.1016/j.diagmicrobio.2009.05.010
Abstrakt: Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis.
Databáze: MEDLINE