| Autor: |
Ting BP; Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore., Zhang J, Gao Z, Ying JY |
| Jazyk: |
angličtina |
| Zdroj: |
Biosensors & bioelectronics [Biosens Bioelectron] 2009 Oct 15; Vol. 25 (2), pp. 282-7. Date of Electronic Publication: 2009 Jul 17. |
| DOI: |
10.1016/j.bios.2009.07.005 |
| Abstrakt: |
This report describes an electrochemical biosensor for the detection of short DNA oligonucleotide of the avian flu virus H5N1 with sequence 5'-CCA AGC AAC AGA CTC AAA-3'. To fabricate this DNA biosensor, a gold (Au) electrode surface was modified with thiolated DNA probes with a sequence complementary to the target DNA. This modified Au electrode was incubated in a buffer solution containing the target DNA to form double-stranded DNA (ds-DNA) through hybridization. The ds-DNA on the electrode surface was then labeled with silver nanoparticles conjugated with a well-known DNA intercalator, doxorubicin. By performing cyclic voltammetry in an aqueous KCl solution (0.3M), the silver nanoparticle labels were detected as a result of the highly characteristic solid-state Ag/AgCl redox process. The signal obtained was subsequently used to quantify the amount of DNA. A detection limit of 1 pM has been achieved with this new DNA biosensor. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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