| Autor: |
Sardela VF; Universidade Federal do Rio de Janeiro, Instituto de Química, Ilha do Fundão, Avenida Athos da Silveira Ramos, 149, 21941-909, LAB DOP-LADETEC, Rio de Janeiro, RJ, Brazil., Motta MT, Padilha MC, Pereira HM, Neto FR |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2009 Oct 01; Vol. 877 (27), pp. 3003-11. Date of Electronic Publication: 2009 Jul 17. |
| DOI: |
10.1016/j.jchromb.2009.07.013 |
| Abstrakt: |
A method for identifying the metabolites of sibutramine 1-(4(chlorophenyl)-N,N-dimethyl-alpha-(2-methylpropyl))cyclobutanemethanamine) in urine, utilizing a double derivatization strategy, with N-methyl-N-(trimethylsilyl)-trifluoroacetamide and N-methyl-bis-(trifluoroacetamide), in gas chromatography/mass spectrometry is proposed. This methodology results in mass spectra with at least three fragments in abundance superior to 20%, attending the World Anti-Doping Agency identification criteria for qualitative assays. The characterization of the derivatives was obtained through two ionization modes: Chemical Ionization and Electron Impact ionization, both in full scan mode. Sibutramine was administered to 5 (five) volunteers and the excretion profile followed for 92h. Routine analytical, hydroxy-cyclobutane-bis-nor-sibutramine which becomes the more abundant metabolite in the first 10h and hydroxy-isopropyl-bis-nor-sibutramine which becomes the most abundant after 40h, were proposed for doping monitoring. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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