The meaning of DAPI bands observed after C-banding and FISH procedures.

Autor: Barros e Silva AE; Laboratory of Plant Cytogenetics, Department of Botany, Federal University of Pernambuco, Recife, PE, Brazil., Guerra M
Jazyk: angličtina
Zdroj: Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 2010 Apr; Vol. 85 (2), pp. 115-25.
DOI: 10.1080/10520290903149596
Abstrakt: Under specific technical conditions chromosome staining with 4',6-diamidino-2-phenylindole (DAPI) permits characterization of heterochromatic regions as AT-rich (DAPI(+)) or AT-poor (DAPI(-)), especially when the chromosomes are counterstained with chromomycin A(3) (CMA), which preferentially binds to GC-rich DNA. DAPI(+) bands also often have been observed after C-banding or FISH. In these cases, however, it is not clear whether only AT-rich regions stain positively with DAPI or other heterochromatins with different base compositions also are stained. We evaluated the meaning of DAPI bands observed after C-banding and FISH using three plant species bearing different types of heterochromatin: DAPI(+)/CMA(-), DAP(-)/CMA(+) and DAPI(0)/CMA(0) (neutral bands). Additional tests were performed using propidium iodide, a fluorochrome without preferential affinity for AT or GC. Our results indicate that AT-rich heterochromatin stains as DAPI(+) bands after C-banding or FISH, but other kinds of heterochromatin also may be stained by DAPI.
Databáze: MEDLINE
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