| Autor: |
Matsumura S; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Ohmori R, Saito H, Ikawa Y, Inoue T |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 2009 Sep 03; Vol. 583 (17), pp. 2819-26. Date of Electronic Publication: 2009 Jul 23. |
| DOI: |
10.1016/j.febslet.2009.07.036 |
| Abstrakt: |
We previously developed a synthetic cis-acting RNA ligase ribozyme with 3'-5' joining activity termed "DSL" (designed and selected ligase). DSL was easily transformed into a trans-acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans-acting DSL, tDSL-1/GUAA. tDSL-1/GUAA exhibited remarkably high activity compared with the parental cis-acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop-receptor interaction plays a significant role in determining the activity of the trans-acting ribozyme and in its ability to perform multiple turnovers of the reaction. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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