| Autor: |
Chen TH; Division of Hog Cholera Research, Animal Health Research Institute 376 Chung-Cheng Road, Tamsui, Taipei County 25158, Taiwan., Pan CH, Jong MH, Lin HM, Huang YL, Hsiung KP, Chao PH, Lee F |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of veterinary medical science [J Vet Med Sci] 2009 Jun; Vol. 71 (6), pp. 703-8. |
| DOI: |
10.1292/jvms.71.703 |
| Abstrakt: |
A chromatographic strip assay was developed for rapid detection of serum antibodies to non-structural protein of foot-and-mouth disease virus. The assay was based on Escherichia coli-expressed 3ABC non-structural protein and an immunochromatographic technique, which shortened the detection time to about one hour. The sensitivity of the assay was determined to be 96.8% for infected pigs; its specificity was 100% for naïve pigs and 98.8% for vaccinated pigs. In the experimentally infected pigs, anti-3ABC antibodies were detectable from eight days post-infection until the end of the study, 34 days post-infection. The performance of this assay was comparable to that of two commercial ELISA kits, Ceditest FMDV-NS and UBI FMDV NS EIA, and was better than that of CHEKIT FMD-3ABC po. Given its advantages of instant testing and quantitative measurement, this assay has potential as a useful tool for rapid on-farm diagnosis of foot-and-mouth disease. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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