Non-HLA T-cell reactivity during the first year after HLA-identical living-related kidney transplantation.
| Autor: | Gerrits JH; Department of Internal Medicine-Transplantation, Erasmus Medical Center-University Medical Center Rotterdam, Rotterdam, The Netherlands., van de Wetering J, Drabbels JJ, IJzermans JN, Claas FH, Weimar W, van Besouw NM |
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| Jazyk: | angličtina |
| Zdroj: | Clinical transplantation [Clin Transplant] 2009 Sep-Oct; Vol. 23 (5), pp. 740-7. Date of Electronic Publication: 2009 Jun 26. |
| DOI: | 10.1111/j.1399-0012.2009.01014.x |
| Abstrakt: | Background: It has been reported that donor-reactive T-cell responses may decrease during the first year after HLA-mismatched organ transplantation. We wondered whether donor-reactive T-cell responses directed to minor histocompatibility antigens (mHAgs) or other non-HLA antigens also decrease after HLA-identical living-related (LR) kidney transplantation. Methods: We studied donor-reactive T-cell responses by IFN-gamma and granzyme B (GrB) Elispot assays in 15 HLA-identical LR kidney transplant recipients before, six months and one yr after transplantation. Third-party reactivity was used as control. Patient and donor peripheral blood mononuclear cells were typed for 11 known mHAgs. Results: During the study period, 60% and 36% of the patients demonstrated donor-reactive IFN-gamma and GrB producing cells (pc), respectively. The number of donor-reactive IFN-gamma and GrB pc was significantly lower than the number of third-party reactive IFN-gamma and GrB pc. After transplantation, donor-reactivity and third-party reactivity were comparable to pre-transplant values. No relation was found in mHAg mismatches between donor and recipient and donor-reactive T-cell response. Conclusions: Donor-reactivity could be detected before and after HLA-identical LR kidney transplantation, but was not related with the number of mHAg mismatches, and did not decrease after transplantation. |
| Databáze: | MEDLINE |
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