Prevalence of Bordetella pertussis and Bordetella parapertussis in Samples Submitted for RSV Screening.

Autor:	Walsh P; Kern Medical Center, Department of Emergency Medicine and David Geffen School of Medicine, University of California Los Angeles., Overmeyer C, Kimmel L, Feola M, Pusavat J, Nguyen TA, Kuan S, Emery K, Rosengreen M, Mordechai E, Adelson ME
Jazyk:	angličtina
Zdroj:	The western journal of emergency medicine [West J Emerg Med] 2008 Aug; Vol. 9 (3), pp. 135-40.
Abstrakt:	Background: The clinical presentation of Bordetella pertussis can overlap with that of respiratory syncytial virus (RSV); however, management differs. Hypothesis: First, the prevalence of B. pertussis is less than 2% among patients screened for RSV, and second the prevalence of B. parapertussis is also less than 2% among these patients. Methods: Nasal washings submitted to a clinical laboratory for RSV screening were tested for B. pertussis and B. parapertussis, using species-specific real-time polymerase chain reaction (PCR) assays. These were optimized to target conserved regions within a complement gene and the CarB gene, respectively. A Bordetella spp. genus-specific real-time PCR assay was designed to detect the Bhur gene of B. pertussis, B. parapertussis, and B. bronchiseptica. RSV A and B subtypes were tested by reverse transcription-PCR. Results: Four hundred and eighty-nine clinical samples were tested. There was insufficient material to complete testing for one B. pertussis, 10 RSV subtype A, and four RSV subtype B assays. Bordetella pertussis was detected in 3/488 (0.6%) (95% CI 0.1% to 1.8%), while B. parapertussis was detected in 5/489 (1.0%) (95% CI 0.3% to 2.4%). Dual infection of B. pertussis with RSV and of B. parapertussis with RSV occurred in two and in three cases respectively. RSV was detected by PCR in 127 (26.5%). Conclusion: The prevalence of B. pertussis in nasal washings submitted for RSV screening was less than 2%. The prevalence of parapertussis may be higher than 2%. RSV with B. pertussis and RSV with B. parapertussis coinfection do occur.
Databáze:	MEDLINE
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