| Autor: |
Baetas-da-Cruz W; Federal University of Rio de Janeiro, Institute of Biophysics Carlos Chagas Filho, Laboratory of Developmental Neurobiology, Rio de Janeiro, Brazil., Alves L, Guimarães EV, Santos-Silva A, Pessolani MC, Barbosa HS, Corte-Real S, Cavalcante LA |
| Jazyk: |
angličtina |
| Zdroj: |
Histology and histopathology [Histol Histopathol] 2009 Aug; Vol. 24 (8), pp. 1029-34. |
| DOI: |
10.14670/HH-24.1029 |
| Abstrakt: |
Complex carbohydrate structures are essential molecules of infectious microbes and host cells, and are involved in cell signaling associated with inflammatory and immune responses. The uptake of mannose-tailed glycans is usually carried out by macrophages, dendritic cells (DCs), and other professional phagocytes to trigger MHC class I- and MHC class II-restricted antigen presentation, and to promote T cell effector responses. Since Schwann cells (SCs) have been proposed as immunocompetent cells, we investigated whether a human cell line (ST88-14 cells) could bind mannosylated ligands in a specific manner. The saturation of uptake of mannosylated molecules by ST88-14 cells and the internalization and distribution pathway of these ligands were tested by cytometry and confocal plus electron microscopy, respectively. This uptake showed a dose-dependent increase, the saturation point being reached at high concentrations of mannosyl residues/240 mM mannose. Merging of man/BSA-FITC and S100 labeling showed their partial, but, significant colocalization. Ultrastructural analysis of ST88-14 cells after incubation with HRP-colloidal gold, without or with subsequent chasing at 37C, showed an initial location on the cell surface and temperature- and time-dependent internalization of the probe. Our findings suggest an efficient mannosylated ligand uptake system through putative lectin(s) that may be operational in inflammatory and immune responses. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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